Postdoctoral or Research Assistant Positions for tRNA Research at Thomas Jefferson University

2018年11月05日

Postdoctoral or Research Assistant positions are available in the lab of Dr. Ya-Ming Hou at Thomas Jefferson University, Sidney Kimmel Medical College.

We study epigenetic post-transcriptional modifications of tRNA and how epigenetic changes on tRNA impact codon-specific translation in diseases. Codon-specific translation is emerging as a new layer that regulates gene expression at the codon level and it is determined by epigenetic modifications to the tRNA anticodon. These modifications are activated or repressed by stress. Current topics include RNA biosynthesis, maturation, epigenetic methylation, and translational activity in mitochondria and in disease.

For more information, please see our website:
Email: このメールアドレスはスパムボットから保護されています。閲覧するにはJavaScriptを有効にする必要があります。
Lab website: https://www.jefferson.edu/university/research/researcher/researcher-faculty/hou-laboratory.html

Candidates with an expected or recent Ph.D. and a strong research productivity are encouraged to apply. Experience in RNA-seq, high-throughput data analysis, fluorescence microscopy, mammalian cell culture, molecular biology, protein enzymology, nucleic acid research, and mitochondrial research is preferred.

Please send a cover letter, a CV, and names/contact information of three references to:
Ya-Ming Hou, Ph.D.
Professor, Department of Biochemistry and Molecular Biology
Thomas Jefferson University
Sidney Kimmel Medical College
233 South 10th Street, BLSB 220
Philadelphia, PA 19107
Email: このメールアドレスはスパムボットから保護されています。閲覧するにはJavaScriptを有効にする必要があります。

Publications:

Masuda, I, Matsubara, R, Christian, T, Rojas, E, Yadavalli, SS, Zhang, LS, Goulian, M, Foster, L, Huang, KC, Hou, YM. (2018) tRNA methylation is a global determinant of  bacterial multi-drug resistance. Cell, in revision.

Gamper, H, Hou, YM. (2018) tRNA 3'-amino-tailing for stable amino acid attachment. RNA, PMID: 30217865.

Masuda, I, Takase, R, Matsubara, R, Paulines, MJ, Gamper, H, Limbach, PA, Hou, YM. (2018) Selective terminal methylation of a tRNA wobble base. Nucleic Acids Res, 46 (7):e37. PMID: 29361055; PMCID:PMC5909439

Oprescu, SN, Chepa-Lotrea, X, Takase, R, Golas, G, Markello, TC, Adams, DR, Toro, C, Hou, YM, Malicdan, MCV, Gahl, WA, Tifft, CJ, Antonellis, A. (2017) Compound heterozygosity for loss-of-function glycyl-tRNA synthetase mutations in a patient with a multi-system and developmental syndrome. Human Mutations 38, 1412-1420. PMID: 28675565; PMCID:PMC 5599332

Masuda, I, Igarashi, T, Sakaguchi, R, Nitharwal, R, Han, KY, Leslie, BJ, Liu, C, Gamper, H, Ha, TJ, Sanyal, S, Hou, YM. (2017) A genetically encoded fluorescent tRNA is active in live-cell protein synthesis. Nucleic Acids Res. 45, 4081-4093. PMID: 27956502; PMCID: PMC5397188

Gall, AR, Datsenko, KA, Figueroa-Bossi, N, Bossi, L, Masuda, I, Hou, YM, Csonka, LN. (2016) Mg2+ regulates transcription of mgtA in Salmonella enterica serovar Typhimurium via prolyl-bond formation during translation of the leader peptide MgtL. PNAS 113, 15096-15101. PMID: 27849575; PMCID: PMC5206563
** Featured in PNAS Commentary: Hughes, KT. (2016) Mg2+-dependent translational speed bump acts to regulate gene transcription. PNAS 113, 14881-14883. PMID: 27994154

Christian, T, Sakaguchi, R, Perlinska, AP, Lahoud, G, Ito, T, Taylor, EA, Sulkowska, JI, Yokoyama, S, Hou, YM. (2016) Methyl transfer by substrate signaling in a knotted protein fold. Nat Struct Mol Biol, 10, 941-948. PMID: 27571175; PMCID:PMC5429141. 
** Featured in Jefferson Research Highlights; ** Selected as an Editor’s Choice in Science “Dynamics of a protein knot”, Science (2016) 354, 194 (http://science.sciencemag.org/content/354/6309/twil);
**Featured in “Math in the Media” column in the American Math Society (www.ams.org/math-in-the-media)(http://www.ams.org/news/math-in-the-media/math-in-the-media)(2016) Dec 1.

Falk, MJ, Gai, X, Shigematsu, M, Vilardo, E, Takase, R, McCormick, E, Christian, T, Place, E, Pierce, EA, Consugar, M, Gamper, HB, Rossmanith, W, Hou, YM. (2016) A novel HSD17B mutation impairing the activities of the mitochondrial RNase P complex causes X-linked intractable epilepsy and neurodevelopmental regression. RNA Biology 13, 477-485. PMID: 26950678; PMCID: PMC4962811

Gamper, HB, Masuda, I, Frenkel-Morgenstern, M, Hou, YM. (2015) Maintenance of protein synthesis reading frame by EF-P and m1G37-tRNA. Nature Communications, May 26;6:7226. doi: 10.1038/ncomms8226. PMID: 26009254; PMCID: PMC4445466

Sakaguchi, R, Lahoud, G, Christian, T, Gamper, H, Hou, YM. (2014) A divalent metal ion-dependent N1-methyl transfer to G37-tRNA. Chem and Biol (Cell Press) 21, 1351-1360. PMID: 25219964; PMCID: PMC4224600

Naganuma, M, Sekine, SI, Chong, YE, Guo, M, Yang, XL, Gamper, H, Hou, YM, Schimmel, P, Yokoyama, S. (2014) The selective tRNA aminoacylation mechanism based on a single G:U base pair. Nature 510, 507-511. PMID: 24919148; PMCID: PMC4323281
**Featured in Nature News and Views: Vargas-Rodriguez, O, Musier-Forsyth, K (2014) Wobble puts RNA on target. Nature 510, 480-481. PMID: 24919145

Frenkel-Morgenstern, M, Danon, T, Christian, T, Igarashi, I, Cohen, L, Hou, YM, Jensen, LJ. (2012) Genes adopt non-optimal codon usage to generate cell-cycle-dependent oscillations in protein levels. Molecular Systems Biology 8, 572. PMID: 22373820

 

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